BAR Barbiturate Drug Test Urine Single Panel

The BAR One Step Barbiturates Test Device (Urine) is an immunoassay based on the principle of competitive binding. Drugs that may be present in the urine specimen compete against the drug conjugate for binding sites on the antibody.
During testing, a urine specimen migrates upward by capillary action. Barbiturates, if present in the urine specimen below the cut-off level, will not saturate the binding sites of the antibody in the test. The antibody coated particles will then be captured by immobilized Barbiturates-protein conjugate and a visible colored line will show up in the test line region. The colored line will not form in the test line region if the Barbiturates level exceeds the cut-off level, because it will saturate all the binding sites of anti-Barbiturates antibodies.
A drug-positive urine specimen will not generate a colored line in the test line region because of drug competition, while a drug-negative urine specimen or a specimen containing a drug concentration less than the cut-off will generate a line in the test line region. To serve as a procedural control, a colored line will always appear at the control line region indicating that proper volume of specimen has been added and membrane wicking has occurred